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. 2017 Nov 23;7:16095. doi: 10.1038/s41598-017-16453-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validity analysis. (A) First, to confirm the presence of genuine MEPs, total neuro-muscular blockade was performed to eliminate myogenic MEP. One normal lamb was administered intravenous curare under orotracheal ventilation. Consequently, MEPs were recorded in all limbs before administration of curare and disappeared afterwards. (B) To confirm the applicability of our methodology in an animal model of spinal cord injury, another lamb underwent transection of the spinal cord. Similarly, MEPs were recorded in hindlimbs before transection of the spinal cord and disappeared afterwards.