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. 2017 Nov 23;7:16101. doi: 10.1038/s41598-017-16239-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RSS1 is a negative regulator of hypocotyl elongation growth. Phenotype analysis of 7-d-old WT (Col-0), homozygous T-DNA insertion mutant rss1 and transgenic overexpression lines (OE1, OE2, OE3) along with vector control (VC) seedlings growing under (a) long day conditions and (b) continuous darkness. The hypocotyls of rss1 mutants were significantly longer than WT while RSS1OE2 seedlings had relatively shorter hypocotyls. (c) Micrograph of hypocotyls of WT, rss1 and RSS1OE2 seedlings (Bars = 20 μm). (d) Quantification of hypocotyl epidermal cell length of WT, rss1 and RSS1OE2seedlings. Values represent the average from two biological replicates each having 15 seedlings and error bars represent SE. (Student’s t-test; P < 0.01; **WT vs. mutant).