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. 2017 Nov 23;7:16174. doi: 10.1038/s41598-017-16279-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Guide-length screen to determine the effect on specificity of a guide-specific system Heatmaps showing varying degrees of knockdown observed via a dual luciferase assay when guides ranging in different lengths are targeted to the wild-type and respective mutant sequence. Specificity bars show knockdown when normalised to the non-specific control, with 100% being maximal knockdown observed. Maximal allele-specificity observed for each mutation indicated with a red arrow.