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. 2017 Nov 23;7:16115. doi: 10.1038/s41598-017-16394-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment of synchronized HCT116 cells with GSK461364 permits re-entry into G1 with 2N DNA only if p53 is present. HCT116-p53+/+ or -p53−/− cells were arrested in 0.1% serum then stimulated to re-enter cell cycle by addition of 10% FBS. Treatments with DMSO (as control, upper panel) or GSK461364 (20 nM, lower panel) were initiated 1 h after serum stimulation. Cells were harvested for flow cytometry analysis at indicated times. Arrows (bottom panel) highlight the re-appearance of cells in G1 in the p53+/+ cells, but not in the p53−/− cells. The data are representative of two independent experiments, each done in triplicate. Error bars represent the standard deviation of the mean.