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. 2017 Nov 23;7:16115. doi: 10.1038/s41598-017-16394-2

Figure 5.

Figure 5

p53 reduces the delay in mitosis resulting from inhibition of PLK1. HCT116-p53+/+ (left panels) and -p53−/− cells (right panels) were treated with DMSO, 5, 10 or 20 nM GSK461364 (A) or DMSO, 10 or 20 nM Taxol (B). Time-lapse microscopy analysis was then used to determine the duration of mitosis. (C) HCT116-p53+/+ and -p53−/− cells were pretreated for 1 h with DMSO or 10 μM KU-55933 and 10 μM VE-82, then subsequently treated with DMSO, 10 or 20 nM GSK461364. Time-lapse analysis was used to determine the duration of mitosis. Graphs show cumulative data from 50 cells for each treatment and are representative of three replicates.