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. 2017 Nov 23;7:16120. doi: 10.1038/s41598-017-16301-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of CSC on U937-cell derived exosomal proteins and antioxidant properties. (a) Coomassie blue staining patterns of exosomes that were derived from DMSO as Control or cigarette smoke condensate (CSC)–exposed U937 monocytic cells. C-Exo: Control-exosome, CSC-Exo: CSC-exosome. Equal amount of protein (10 µg) was loaded in each well. (b) Western blot of exosomal marker proteins CD63, Alix, and CD81 and cellular marker protein actin. Equal amount of protein (10 µg) was loaded in each lane. C-CE: control-cell extract, CSC-CE: CSC-cell extract, C-Exo: Control-exosome, CSC-Exo: CSC-exosome. (c) Total protein levels of exosomes that are quantified using BCA protein estimation method. Bars indicate mean ± SEM values from three replicates. (d) Total antioxidant capacity of exosomes measured by total antioxidant capacity assay. *Indicates p < 0.05 compared to control.