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. 2017 Nov 23;7:16123. doi: 10.1038/s41598-017-16331-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing effect of target genes dsRNAs on different days (1^st, 3^rd, 5^th & 7^th) at different concentrations. Feeding by leaf dip method against target genes (PcVg & PcVgR) (A & B) at different concentrations (250, 500, 750 & 1000 ng/ul) were analyzed. Target gene expression is compared relative to 1 fold of ds-EGFP control. Error bars represent the SE of the mean of three independent biological replicates. All treatments were analyzed as compared to control by using ANOVA Tukey’s-test, P < 0.0001.