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. 2017 Nov 23;7:16123. doi: 10.1038/s41598-017-16331-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Egg laying and hatching rate in response to combinations of target genes (PcVg + PcVgR) dsRNA compared with control group (ds-EGFP). Three individual biological replicates were performed, *** indicates the significant difference. One way ANOVA indicate the difference in an average number of laying between all candidate genes dsRNA (P < 0.0001, Tukey test).