Fig. 3.

Rap1b recognizes F0 domain of talin in a GTP-dependent manner. a The HSQC spectra (representative regions were shown) of 45 μM GMP-PNP loaded ¹⁵N-labeled Rap1b (1–167) in the absence (black) and presence of 90 μM talin-F0 (red), 225 μM talin-F0 (blue), 90 μM talin-H (red), or 45 μM full-length talin (red). Note that the peak of I27 was broadened in the presence of talin-H or full-length talin. b GST pull-down assays to show that Rap1b interacts with both talin-H and full-length talin in a GTP-dependent manner. Full blot/gel images are shown in Supplementary Figs. 11 and 12