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. 2017 Nov 23;7:16102. doi: 10.1038/s41598-017-16487-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression assays. Quantitative RT-PCR gene expression assays (qReal Time TaqMan probes PCR) in white adipose tissues of Tg and control littermate mice fed with chow or HFD, upon sacrifice at 7-month of age. Relative expression of MCP-1, Cd68, IL-6 and TNF-α pro-inflammatory genes are given in fold change (FC) 2^−ΔΔCt. Peptidyl-prolyl isomerase A (CypA) probe was used as endogenous control (n = 8 per group), #p < 0.05 chow vs HFD. Results correspond to mean values from three independent set of experiments including n = 8 animals/group. For all panels error bars correspond to SEM.