Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 23;7:16179. doi: 10.1038/s41598-017-15532-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Altered phosphorylation signatures observed in a latently infected Jurkat cell line model following T cell activation. (a) Schematic depicts the sorting strategy for generating a clonal DHIV-GFP/ HIV-1 _LAI (X4)-infected Jurkat cell line (J-DHIV). (b) Histogram of GFP expression in the J-DHIV cells before (black) and after stimulation with PMA/I (yellow) measured by flow cytometry. (c) Viral reactivation levels after 24 hours of stimulation across a panel of LRAs. Data presented as means ± SD for 3 biological replicates. (d,e) Heatmaps depict phospho-protein time courses following CD3/CD28 or PMA/I stimulation in Jurkat and J-DHIV cells over 24 hours measured by bead-based immunoassay. Phospho-signals were measured in biological triplicate and 90% of measurements had an SD < 14%. Data are normalized to the maximum signal for each paired stimulation time course (uninfected and infected cells). Raw data are provided in Supplementary Datasets S7–S8. (f–g) Flow cytometry analysis of p-ERK at 15 minutes (f) and p-p38 at 24 hours (g) in uninfected Jurkat, latently infected (GFP-) and infected/reactivating (GFP+) J-DHIV cells following CD3/CD28 (top) or PMA/I stimulation (bottom). Gating for p24 (left plots) and histogram intensities for each population (middle plots) are shown. MFI of phospho-protein levels (right plots) presented as means ± SD for 3 biological replicates.