Fig. 1.

PDGF-AB enhances cell migration through microporous membranes and tissue. a Schematic of Boyden chamber chemotaxis assay, with platelet-derived growth factor-AB (PDGF-AB) as the chemoattractant. b Confocal top–down and cross-sectional views showing adult meniscal cells (green) passing through microporous membranes (red) with 3 and 8 µm diameter pores. Arrow highlights constricted nucleus (blue). Scale = 10 µm. c Migrated cell signal intensity normalized to the 3 µm pore group for each media condition (n = 4 samples/group, mean ± s.d.). d Schematic of a tissue Boyden chamber. A devitalized fetal tissue substrate (blue) is interposed between a glass slide with communicating holes and a chemokine reservoir. Fluorescently labeled cells migrate from an adult tissue explant and through the tissue along the chemotactic gradient, established by the underlying reservoir. e Confocal top–down and cross-sectional views showing cells migrating through the tissue barrier. Arrows point to representative migrated cells. Scale = 20 µm. f Total number and migrated cells for each media condition (n = 3 samples/group, mean ± s.d.). * = p < 0.05 vs. Control, ^# = p < 0.05 vs. 3 µm pore