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. 2017 Nov 24;7:16214. doi: 10.1038/s41598-017-15376-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Isolation and characterization of extracellular vesicles isolated from murine BM-MSCs. (A) Experimental protocol for isolation of microparticles (MPs) and exosomes (Exos) using differential ultracentrifugation. (B) Size of MPs (up) and Exos (down) detected in 200 µL by Dynamic Light Scattering analysis (C) Number and size of MPs (up) and Exos (down) detected in 1 mL (corresponding to 1 µg EV equivalent proteins) by Nano Tracking Analysis. (D) Representative images of Exos and MPs by transmission electron microscopy. (E) Expression of BM-MSC membrane markers (Sca-1, CD44, CD29) and of exosomal markers (CD9, CD81) on MPs (top) and Exos (bottom) isolated from naïve BM-MSCs as analysed by flow cytometry.