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. 2017 Nov 24;7:16229. doi: 10.1038/s41598-017-16231-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of 3A8-C12 MAb reactivity. (A) Indirect immunofluorescence of YFV/17DD-infected Huh7.5 cells (confocal microscopy) stained with the 3A8-C12 monoclonal antibody plus anti-mouse IgG conjugated with AlexaFluor 488. DNA was stained using DAPI; (B) Western blotting analysis of rNS1 resolved by 15% SDS-PAGE. The membrane was stained with the 3A8-C12 monoclonal antibody, followed by anti-mouse IgG conjugated with alkaline phosphatase. Solid arrows indicate oligomeric forms of YFV rNS1: (M) monomers, (D) dimers and (T) trimers.