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. 2017 Nov 24;7:16229. doi: 10.1038/s41598-017-16231-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of the cross-reactivity of the 3A8-C12 MAb against different flaviviruses. Huh7.5 cells were infected with YFV, DENV, SLEV, and WNV and then immunostained. The 4G2 MAb against the Flavivirus E protein was used as a positive control, and uninfected cells (MOCK) were used as the negative control. The plotted data are representative of two independent experiments. The cell suspensions were analyzed by flow cytometry on a FACSCanto II (BD Biosciences, San Jose, California, USA). All flow cytometry assay data were analyzed in FlowJo v7.1 (TreeStar Inc., Ashland, Oregon, USA).