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. 2017 Nov 24;7:16229. doi: 10.1038/s41598-017-16231-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetics of cell infection and NS1 production and secretion. Percentage of infected cells (left panel) and dosage of NS1 in cell lysates (middle panel) and culture supernatant (right panel) from (A) Huh7.5 cells infected with YFV vaccine strain (17DD); (B) Huh7.5 cells infected with YFV wild strain (M17/09); and (C) C6/36 cells infected with YFV wild strain (M17/09), all at MOI of 0.125. All sNS1 measurements under the limit of sensibility were considered negative (ND). Statistical analysis performed with unpaired t-test with Welch’s correction: ns if p > 0.05; * if p ≤ 0.05; ** if p ≤ 0.01; *** if p ≤ 0.001. Data represent the mean ± SD of three technical replicates.