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. 2017 Nov 24;7:16261. doi: 10.1038/s41598-017-16484-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amount of viral RNA in DEF cells overexpressing WT PABP, the Q367N cleavage-resistant variant and truncated PABP. (a) DEF cells overexpressing WT PABP or the Q367N cleavage-resistant variant were infected with DHAV. At 12 hpi, total RNA was isolated and the copy numbers of VP0 RNA were detected with a one-step rRT-PCR assay and compared with those cells transfected with the empty vector. (b) DEF cells expressing the N-terminal domain (NTD) of PABP and C-terminal domain (CTD) of PABP were infected with DHAV. At 12 hpi, total RNA was isolated and the copy numbers of VP0 RNA were detected and compared with those cells transfected empty vector. *P < 0.05; ****P < 0.0001. (c) The cell lysates in the same batch of cells expressing WT PABP and the Q367N cleavage-resistant variant were analysed by immunoblotting. Cell lysates were detected with anti-Flag antibody. (d) The lysates of cells expressing NTD of PABP and CTD of PABP were analysed by immunoblotting. Cell lysates were detected with anti-Flag antibody.