Skip to main content
. 2017 Nov 24;7:16286. doi: 10.1038/s41598-017-16490-3

Figure 3.

Figure 3

Inhalation exposure to PM2.5 induces autophagy in the livers of mice under NC or HFD. (A) Western blot analyses of levels of LC3 and P62 in the liver tissues from NC- or HFD- fed mice exposed to PM2.5 or FA for 10 weeks. Levels of β-actin were determined as loading controls. The graphs beside the images show the quantitative analyses of fold changes of ratios of LC3-II vs LC3-I and P62 protein levels, as determined by Western blot densitometry, in the livers of NC- or HFD- fed mice exposed to PM2.5 or FA. The protein level was normalized to that of β-actin before fold change calculations. Each bar denotes mean ± SD. *p < 0.05. (B–G) Expression levels of Tfeb, Lc3b, Psap (Prosaposin), Gba (Glucosidase, beta, acid), Csta (Cystatin A), and P62 mRNAs in the livers of NC- or HFD- fed mice exposed to PM2.5 or FA for 10 weeks. Expression levels of mRNAs were determined by qPCR. Fold changes of mRNA levels are shown by comparing to that of one of NC-fed mice under FA exposure. Each bar denotes mean ± SEM (n = 4 mice per group). *p < 0.05, **p < 0.01. (H) BODIPY staining of lipid droplets in livers of NC- or HFD- fed mice exposed to PM2.5 or FA for 10 weeks (magnification: 200×).