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. 2017 Nov 24;7:16319. doi: 10.1038/s41598-017-16641-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell cultivation, membranes and pigment binding complexes isolation. Panel a: H. pluvialis cultures grown under 3 different stress conditions. Green: 50 µmol m⁻²s⁻¹ for 7 days; Orange: 400 µmol m⁻²s⁻¹ for 3 days; Red: 400 µmol m⁻²s⁻¹ for 6 days. Panel b: microscope observation of cells grown as in Panel A. Panel c: isolation of plastid membranes from G, O and R cells. Purified membranes are indicated by the arrow. Panel d: Sucrose gradient ultracentrifugation separation of pigment binding complexes from plastid membranes solubilized in β-DM 1%.