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. 2017 Nov 24;7:16319. doi: 10.1038/s41598-017-16641-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Singlet oxygen production and chlorophyll photo-bleaching in isolated membranes. Panel a: singlet oxygen production indirectly determined following the increase of fluorescence of Singlet Oxygen Sensor Green (SOSG), a fluorescent probe increasing its fluorescence in presence of singlet oxygen. Isolated membranes were illuminated with red light at 6000 µmol m⁻²s⁻¹. All data were normalized to chlorophyll content and to the SOSG fluorescence of green membranes after 30′ of illumination. Panel b: chlorophylls photo-bleaching induced in isolated membranes upon illumination with white light at 6000 µmol m⁻²s⁻¹. Standard deviations are indicated in both panels (n = 3).