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. 2017 Nov 24;7:16217. doi: 10.1038/s41598-017-16522-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Formation of P. gingivalis 33277-S. gordonii DL1 heterotypic biofilms. S. gordonii DL1 biofilms were established on polystyrene surfaces coated with human whole saliva. P. gingivalis 33277 grown with or without SAPP (24 µM) was reacted with the DL1 biofilms for 4 h. The bound 33277 in the biofilms was quantitated using qPCR. Each bar represents the number of 33277 cells detected in the biofilms. Error bars indicate standard deviations.