Figure 1.

Fitting to different scan rates alone fails to account for reheating. (A) Global fitting of model B (blue) to DSC data (black) for denaturation of the DhaA115 mutant while disregarding reheated runs. The scan rates were 0.5 (◽), 1 (○) and 2 °C min⁻¹ (◊). (B) Resulting reheated runs (pink) and actual reheated runs (black crosses) for a scan rate of 1 °C min⁻¹. (C) Global fitting of model D (blue) to DSC data (black) for denaturation of the DhaA mutant with reheated runs. The scan rates were 0.5 (◽), 1 (○) and 2 (◊)°C min⁻¹. (D) Resulting reheated runs (pink) and actual reheated runs (black crosses) for a scan rate of 1 °C min⁻¹.