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. 2017 Nov 24;7:16321. doi: 10.1038/s41598-017-16360-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The complex three-step modelling of thermal unfolding of wild type DhaA. (A) DSC data (black) for the denaturation of DhaA wild type: first runs (circles), reheated runs for terminal temperatures 49, 51, 54, 61, and 69 °C (not shown), fitted curves for the first run (blue) with decomposition by peaks (dotted) from model C plus one negative peak at high temperatures. (B) Respective modelled fractions of states for a given temperature: native folded (black), first intermediate (yellow), second intermediate (brown) and denatured (red) states. The scan rate was 1 °C min⁻¹.