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. 2017 Nov 24;7:16280. doi: 10.1038/s41598-017-16139-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic analysis of HA and NA genes. Phylogenetic trees of the HA (A) and NA (B) genes were constructed using the distance-based neighbor joining method in MEGA7.0.21 software. The reliability of the trees was assessed by bootstrap analysis. Horizontal distances are proportional to genetic distances. The H5N6 viruses analyzed in the present study are indicated in blue. Human H5N6 viruses are indicated in red.