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. 2017 Nov 24;7:16280. doi: 10.1038/s41598-017-16139-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agglutination activities of the H5N6 viruses for various red blood cells. Four types of red blood cells: a Chicken red blood cells (with α-2,3-linked sialic acid receptors and α-2,6-linked sialic acid receptors). b, Chicken red blood cells treated with α-2,3-sialidase (with only α-2,6-linked sialic acid receptors). c, Sheep red blood cells (with only α-2,3-linked sialic acid receptors). d, Chicken red blood cells treated with VCNA (no receptors). Figure 2A,B and C show the agglutination activities of DK01, CK918 and CK165 for the four types of red blood cells, respectively. Figure 2D represents the HA titers of DK01, CK918 and CK165 in the four types of red blood cells.