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. 2017 Nov 24;7:16280. doi: 10.1038/s41598-017-16139-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Horizontal transmission of the H5N6 viruses between guinea pigs. Groups of three guinea pigs were inoculated with the indicated viruses. The next day, the inoculated animals were paired by co-housing with direct-contact guinea pigs; aerosol-transmission animals were also housed in a wire-frame cage adjacent to the infected guinea pigs. Nasal washes were collected from all the animals for virus shedding detection every other day beginning on day 2 after the initial infection. Each color bar represents the virus titer in an individual animal. Dashed lines indicate the lower limit of virus detection.