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. 2017 Nov 24;7:16226. doi: 10.1038/s41598-017-16373-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NGR1 reduced cell apoptosis via PLC. (A) The MTT assay showed that m-3M3FBS pretreatment abolished the neuroprotective effects of NGR1, and there was lower cell viability with m-3M3FBS treatment in the OGD/R + NGR1 + m-3M3FBS group than in the OGD/R + NGR1 group; m-3M3FBS treatment independently aggravated cell injury. (B) LDH leakage was higher in cells exposed to m-3M3FBS than in the OGD/R + NGR1 group; m-3M3FBS treatment independently aggravated LDH leakage. (C and D) TUNEL-positive nuclei are indicated by green fluorescent staining, and total nuclei are indicated by blue DAPI staining. M-3M3FBS significantly blocked the neuroprotective effects of NGR1. There were more TUNEL-positive cells in the OGD/R + NGR1 + m-3M3FBS group than in the OGD/R + NGR1 group. m-3M3FBS treatment alone led to more TUNEL-positive cells, and U73122 could inhibit cell apoptosis after OGD/R. Scale bar = 100 μm, *p < 0.05 compared with the OGD/R groups, ^# p < 0.05 compared with the OGD/R + NGR1 groups, n = 5, data presented as mean ± SD.