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. 2017 Nov 24;7:16202. doi: 10.1038/s41598-017-16296-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-PCR results from transfection of wild-type SRD5A2 or c.548-44 T > G SRD5A2 minigenes. A representative agarose gel (n = 3) showing the results of RT-PCR of wild-type (WT) and mutant (MUT) minigene splicing is shown and quantified for the accompanying graph. Schematic diagrams identifying the wild-type transcript (369 bp), a transcript with deletion of 112 nucleotides (257 bp), and a third transcript with exon 4 skipping are shown (217 bp). The panel on the right shows electropherograms of cDNA splicing isoforms generated from wild-type and mutant minigenes. The histogram shows the relative percentage expression of each isoform calculated against total intensity of the bands representing each isoform. Student t test analysis shown; *p < 0.01.