Table 1.
Functional annotation enrichments for gene clusters. Gene clusters described in Fig. 2 were analysed for Gene Ontology term enrichment (GO terms) using flymine online database (http://www.flymine.org/). Significant P values (cutoff 0.05) are indicated (see Supplementary Table S3 for details) and the number of genes associated to corresponding GO terms is displayed (Nb genes/GO Term). TF-binding motifs enriched in non-coding regions of gene clusters were identified using i-cisTarget (https://gbiomed.kuleuven.be/apps/lcb/i-cisTarget/). The predicted corresponding TF and the NES score of the most representative motifs (cut off 5) are indicated (see Supplementary Table S4 for details).
| # Cluster | Timepoint | Nb Genes/Cluster | GO Terms (P.val) | Nb Genes/GO Term | TF predicted (NES score) |
|---|---|---|---|---|---|
| 1 | 30 min | 54 | Response to stress [GO:0006950] (2.10e−3) | 18 | Tbp (10.7) Hsf (8.6) |
| 2 | 30 min | 360 | Antibacterial humoral response [GO:0019731] (2.79e−5) | 12 | — |
| 3 | 30 min | 84 | Eggshell formation [GO:0030703] (1e−7) | 12 | Tbp (7.5) |
| Multi-organism process [GO:0051704] (1.9e−4) | 26 | Taf1 (7.5) | |||
| Dl (5.8) | |||||
| 4 | 30 min | 147 | Tissue development [GO:0009888] (0.03) | 29 | Ken (9.7) Blimp-1 (8.8) Hsf (6.2) |
| 5 | 3 h | 355 | Response to stress [GO:0006950] (0.02) | 52 | Atf3 (5.1) Kay (6.4) Jra (6,4) |
| 6 | 3 h | 416 | Egg coat formation [GO:0035803] (4.56e−7) | 9 | Pnr (8.6) |
| Transmembrane transport [GO:0055085] (2.41e−6) | 39 | GATAe (8.5) | |||
| Immune response [GO:0006955] (0.03) | 26 | Srp (7.5) | |||
| Grn (7.0) | |||||
| 7 | 3 h | 15 | — | — | — |
| 8 | 6 h | 160 | Defense response [GO:0006952] (2.27e−4) | 19 | Tbp (6.3) |
| Rel (6.3) | |||||
| 9 | 6 h | 158 | Egg coat formation [GO:0035803] (4.05e−13) | 10 | — |
| 10 | 6 h | 131 | Cell-cell signaling [GO:0007267] (7.82e−7) | 25 | — |