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. 2017 Nov 24;7:16250. doi: 10.1038/s41598-017-16442-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A temperature change shifts the ACDAN GP out of the oscillating region and destroys the oscillations. Oscillations in (A) NADH autofluorescence and (B) ACDAN GP in BY4743 wild type cells grown at 30 °C. The temperature was initially 25 °C. Oscillations were induced by the addition of first 30 mM glucose (arrow) and 60 s later 5 mM KCN as indicated by the arrows. At approximately 900 s, the temperature was changed from 25 °C to 35 °C.