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. 2017 Nov 24;7:16250. doi: 10.1038/s41598-017-16442-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Iodoacetate eliminates oscillations and induces an increase in ACDAN GP. Oscillations in NADH (A) and ACDAN GP (blue curve in B) following addition of glucose and KCN to a 10% (w/v) suspension of BY4743 WT cells. At approximately 1100 s, 20 mM iodoacetate (IAA) was added to the suspension. The green curve in B represents cells that were incubated with ACDAN and iodoacetate for 1 h before glucose and KCN were added to the suspension. The ACDAN spectra in (C) represent resting cells before addition of glucose and KCN (green), oscillating cells (blue) and cells inhibited with iodoacetate, recorded 1.5 h after the addition of the inhibitor (red curve) The corresponding ACDAN GP values are listed in the box. Yeasts were grown at 30 °C. Oscillations were induced by addition of first 30 mM glucose and 60 s later 5 mM KCN. The measurement temperature was 25 °C.