Fig. 2.

Lipid accumulation is dependent on glucose, but independent of glutamine. a Photomicrographs of Oil Red O-stained 786-O cells cultured in the presence or absence of serum and glutamine, as indicated. Bar = 10 μm. b Crystal violet staining of rescued cells from treatment as in a. Bar = 1 cm. c Photomicrographs of Oil Red O-stained 786-O cells cultured in indicated glucose concentrations. d Quantification of Oil Red O of cells in c and graphed versus glucose concentration. Square of the Pearson correlation coefficient r is shown. e Quantification of Oil Red O of 786-O cells with media changes 0, 2, or 3 times during the 7-day period. f Fluorescent labeling of BrdU incorporation of 786-O cells at subconfluent or at 7 days post confluence. Bar = 10 μm. p values of two-tailed Student’s t tests are displayed. Error bars represent standard deviations