Fig. 4.

CPT1A repression controls lipid deposition in ccRCC cells. a Western blot of RCC4 cell lysates expressing shRNA to HIF1α and HIF2α decorated with antibodies to HIF1α, HIF2α, CPT1A, and β-actin. b Photomicrographs of RCC4 cells with HIF1α and HIF2α knockdown stained for Oil Red O. c Quantification of Oil Red O staining of cells in b. d CPT1A activity measurement in RCC4 and RCC4 VHL mitochondria. e Quantitative real-time PCR of CPT1A (qRTPCR) expression in RCC4 VHL cells expressing three different CPT1A shRNA constructs. f Photomicrographs of Oil Red O-stained RCC4 VHL cells with CPT1A knockdown. Bar = 10 μm. g Quantification of cells in e. h Quantification of CPT1A and GLTU1 expression in subconfluent, confluent, or superconfluent 786-O and RCC4 cells. Error bars represent standard deviations. p values of two-tailed Student’s t tests are displayed