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. 2017 Jul 20;3(2):73–84. doi: 10.1159/000477762

Fig. 3.

Fig. 3

THC-induced alteration in gene expression in hiPSC forebrain neurons from cases with schizophrenia and healthy controls. a Schematic of directed differentiation from hiPSCs. b–d Real-time PCR analysis of GRIA1, GRIA2, GRIN2A, GRIN2B, CNR1, and COX2 expression in hiPSC forebrain neurons treated with DMSO (control), 1 μM THC for 24 h, and 50 nM THC for 7 days. Ca–Cd indicate neurons induced from 4 different individuals (controls a–d). Values are expressed as mean ± SEM, relative to DMSO-treated levels for Ca. e–g Real-time PCR analysis of GRIA1, GRIA2, and CNR1 expression in hiPSC forebrain neurons treated with DMSO (control) or 1 μM THC for 24 h. Ca–Cc indicate neurons induced from 3 controls and Sa-Sc from 3 patients with schizophrenia (controls and schizophrenia patients a–c). Values are expressed as mean ± SEM, relative to DMSO-treated levels for Ca. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. p SZ-CNR1 acute vs. chronic = 0.0008. USA). Data are presented as the mean ± SEM and were analyzed by two-way ANOVA with the Sidak multiple comparison test or Friedman test with Dunn's multiple comparisons test. Values are expressed as mean ± SEM. p > 0.05 (ns); * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.