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. 2017 Nov;3(6):a001495. doi: 10.1101/mcs.a001495

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© 2017 Carter et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse and redistribution provided that the original author and source are credited.

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Figure 3. — Multimodal analysis of FGFR2 in the tumor. (A) Targeted NGS-based virtual karyotype (CNVkit) showing background aneuploidy and an amplification event involving a focal region of Chromosome 10 (Chr10:122737302–123454446) that includes FGFR2. (B) Cytoscan microarray output from the tumor tissue revealing amplification of the region involving FGFR2. (C) Fluorescence in situ hybridization of tumor-involved (Tumor) and tumor-uninvolved (Control) colonic tissue from the patient's colectomy. FGFR2 probe (RP11–62L18, red) to Chromosome 10 enumeration probe (CEP 10, green) ratio showing a high copy-number ratio of 24.2 in the tumor (right) versus 1.92 in the control (left).