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Filipin stain of nonesterified cholesterol in fibroblasts with (A–C) and without (D–F) stimulation of LDL cholesterol uptake. (A,D) Normal control showing 100% esterification. (B,E) Known NPC 1 patient showing 1% esterification. (C,F) Index patient showing 2% esterification. Magnification, 20×.
