Figure 2. Infection with Pseudomonas prolyl hydroxylase (PPHD) knockout P. aeruginosa carries higher mortality.

C57/BL6 mice were instilled intratracheally with 1×10 ⁷ cfu of PA01 wildtype (WT) or PPHD knockout (MT) P. aeruginosa. ( A) Survival was recorded for 5 days post infection, n=10 mice per group. ( B) At 12 hours post infection lungs were harvested and viable bacteria count calculated, n=8 mice per group *p<0.05. Bronchoalveolar lavage (BAL) total cell count ( C), % neutrophils ( D), neutrophil count ( E), macrophage count ( F) and BAL supernatant immunoglobulin M (IgM) ( G) were measured at timepoints from 12–48 hours post infection, n= 4–7 mice per group, *p<0.05, ***p<0.001, ****p<0.0001. ( H) H+E staining of lung tissue taken at 36h after infection (arrows point to neutrophils, x20 magnification). Images representative for n=2 mice per group. ( I) BAL was harvested and the cell pellets analysed by flow cytometry for apoptosis at 12 hours post infection, n=8 mice per group **p<0.01.