Figure 3. Expression profiles of bone sialoprotein (BSP) during osteogenic differentiation by test materials in mPDL cells assayed via RT-PCR and quantitative real-time PCR. (A) RT-PCR results. (B) The relative expression of BSP genes normalized against a housekeeping gene (ß-actin). The mRNA level of osteogenic gene increased in the MTA mixed with 1% and 2% MC/CaCI2. *p<.05, compared to the control.
