Figure 4. Alkaline phosphatase (ALP) staining and alizarin red staining in mPDL cells exposed to extracts of the tested materials. (A) Phenotype expression of ALP during osteogenic differentiation by the tested materials in mPDL cells at 5 d. (B) Quantified data. (C) Matrix mineralization of mPDL cells observed by staining calcium deposits in the extracellular matrix after 14 d. (D) Quantified data. We observed a higher ALP expression and mineralization in cells exposed to tested materials comparatively with the control. *p<.05, compared to the control.
