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. 2017 Nov 10;11:628. doi: 10.3389/fnins.2017.00628

Figure 1.

Figure 1

Differentiation of iPSCs into iPS-NSCs. iPSCs were expanded to 80% confluency, and then, the iPSC media were replaced by neuronal induction media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, and 5 mg/mL streptomycin and 5 UI/mL penicillin). The cells were kept in culture until they detached and formed neurospheres. The media containing detached cells were centrifuged, and the pellet was dissociated in Accutase. Cells were then re-plated in neural stem cell media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, 20 ng/mL EGF, 10 ng/mL bFGF, and 5 mg/mL streptomycin and 5 UI/mL penicillin). Cells were passaged three times and then characterized using ICC.