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Purification and FI based dsRNA formation assays of truncated NS5B. (A) Coomassie stained SDS-PAGE gel analysis of purified truncated NS5B with 6× His tags. (B) Truncated NS5B catalyzed dsRNA formation (adding H2O or 300 nM truncated NS5B in reaction system). (C) The effect of truncated NS5B concentration on dsRNA formation measured using the PicoGreen. (D) About 800 small molecular compounds and crude extracts were screened using the FI system.
