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. 2017 Nov 2;11:341. doi: 10.3389/fncel.2017.00341

FIGURE 1.

FIGURE 1

Effect of noradrenaline (NA) on the neuronal excitability of layer V medial prefrontal cortex (mPFC) pyramidal neurons in the absence of TTX. (A) IR-DIC image of a typical PFC slice with layer V pyramidal neurons (marked with white arrows). (B) Membrane potential depolarization evoked by bath application of NA (50 μM) recorded in the current-clamp classical whole-cell configuration. (C) Representative traces obtained from one neuron showing the response to a depolarizing current step (+150 pA, 1000 ms) before (control, a), during (NA 50 μM, b) and after NA bath application (washout, c). (D) Mean number of spikes evoked by depolarizing current steps (from +50 to +350 pA in 50 pA increments) before (control, open circles), during (NA 50 μM, black circles) and after (washout, gray circles) NA bath application. Horizontal arrows shown in this and other figures indicate the resting membrane potential or control holding current level. Continuous horizontal bars above the recording traces indicate the bath application of the agonists in this and other figures.