FIGURE 11.

Effect of βγ subunit-dependent signaling inhibitors on the β₁-adrenergic receptor-dependent inward current in layer V mPFC pyramidal neurons. (A) Inward currents evoked by bath application of dobutamine alone (DOB, 100 μM, a) and dobutamine (DOB, 100 μM) in the presence of gallein (20 μM in the bath, b). Amplitude of the holding current change evoked by 100 μM dobutamine alone (DOB) and 100 μM dobutamine in the presence of 20 μM gallein (+ gallein, c). The slices were also exposed to gallein in the extracellular solution for 2 h before current recordings. (B) Inward currents evoked by bath application of dobutamine alone (DOB, 100 μM, a) and dobutamine (DOB, 100 μM) in the presence of GRK2i (10 μM in the pipette solution, b). Amplitude of the holding current change evoked by 100 μM dobutamine in the absence (DOB) and presence of GRK2i 10 μM (+ GRK2i) (c). The currents were recorded >50 min after obtaining access to the cell; ^∗p < 0.05, ^∗∗p < 0.01.