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. 2017 Nov 2;11:341. doi: 10.3389/fncel.2017.00341

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Copyright © 2017 Grzelka, Kurowski, Gawlak and Szulczyk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of the phospholipase C (PLC) and protein kinase C (PKC) inhibitors on the β₁-adrenergic receptor-dependent inward current in layer V mPFC pyramidal neurons. (A) Inward current evoked by bath application of dobutamine (DOB, 100 μM) in the presence of the PLC inhibitor (U 73122, 10 μM in the bath and 10 μM in the pipette, a). Amplitude of the holding current change evoked by 100 μM dobutamine alone (DOB) and 100 μM dobutamine in the presence of 10 μM U 73122 in the bath and 10 μM in the pipette (+ U 73122, b). (B) Inward current evoked by bath application of dobutamine (DOB, 100 μM) in the presence of the PKC inhibitor (chelerythrine, 10 μM in the bath and 10 μM in the pipette, a). Amplitude of the holding current change evoked by 100 μM dobutamine alone (DOB) and 100 μM dobutamine in the presence of 10 μM chelerythrine in the bath and 10 μM in the pipette (+ chelerythrine, b); n.s., non-significant.