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. Author manuscript; available in PMC: 2018 Feb 9.
Published in final edited form as: Pediatr Res. 2017 Aug 9;82(6):1022–1029. doi: 10.1038/pr.2017.175

Figure 3. Bmp4 expression and canonical signaling is reduced in E11.5 Frs2αST−/− peri-Wolffian duct stroma.

Figure 3

(a–b) Representative whole-mount in situ hybridization in urogenital ridges reveals that compared to the control (a), Bmp4 expression is virtually absent adjacent to the Wolffian duct (arrowheads) in Frs2αST−/− embryos (b, arrowheads). (c–d) In contrast to Bmp4, in situ hybridization reveals similar Ret expression in Wolffian ducts (arrowheads) of the control (c) and mutant (d). (e) Representative Western blot analysis for pSmad 1/5/8 (pSmad) expression in control and Frs2αST−/− urogenital ridges suggests reduced canonical Bmp signaling in Frs2αST−/− tissues. (f) Ratios of pSMAD 1/5/8 and β-actin loading control bands quantified by densitometry confirms a significant reduction in canonical Bmp4 signaling. Dotted lines encircle the metanephros. (n) = 3. Values = Mean + SD. Student’s Test = *p<0.05. Scale bar = 200μm.