Skip to main content
. Author manuscript; available in PMC: 2017 Nov 24.
Published in final edited form as: Biochemistry. 2017 May 4;56(19):2506–2517. doi: 10.1021/acs.biochem.6b01245

Figure 2.

Figure 2

Separation of P-gp-embedded nanodiscs from empty nanodiscs. (A) SEC–HPLC elution profile of the nanodisc reconstitution mixture (blue) and purified P-gp nanodiscs (red). (B) Visualization of eluted fractions of the nanodisc reconstitution mixture from 15 to 28 min, by Coomassie staining of reducing SDS–PAGE. Fractions containing P-gp that co-eluted with histidine tag-cleaved MSP1D1 were pooled together (fractions 17–20) and concentrated using a 100 kDa MWCO spin filter.