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. Author manuscript; available in PMC: 2017 Nov 24.
Published in final edited form as: Biochemistry. 2017 May 4;56(19):2506–2517. doi: 10.1021/acs.biochem.6b01245

Figure 3.

Figure 3

Modulation of ATPase activity of P-gp by drugs and fluorescent analogues. P-gp DMPC nanodiscs (0.5 μg), in the presence of 1 mM ATP, were used for ATPase activity assays with ligand: (A) (○) verapamil and (■) BD-verapamil, (B) (○) vinblastine and (■) BD-vinblastine, and (C) (○) paclitaxel and (■) Flutax-2. (D) Effect of fluorescent probes on the ATPase activity of P-gp reconstituted into E. coli nanodiscs. See ref 16 for details about E. coli lipid nanodisc reconstitution. Each data point represents the mean ± standard deviation of triplicate measurements.