Fig.8.

In vivo axonal transport recovery in spinal cord of animal transplanted or not with Er-NPCs. Panel A. Qualitative image of anterograde axonal transport at 25 days after lesion in PM-NPC treated animals. As described in material and methods section fluororuby was injected at T6/T7 at day 20 after lesioning and animal sacrificed five days later. Schematic reconstruction of spinal cord longitudinal sections of lesioned (below) and lesioned+Er-NPCs (above) treated animals. Er-NPCs were stained with Hoechst (blue). Panel B. Quantification of fluorescence 2 cm, 1 cm and 1 mm away from the lesion. Sections were taken from animals transplanted or not with Er-NPCs. Quantification was performed in three animals per group 25 days after lesion. We determined the statistical differences by means of an ANOVA test followed by Bonferroni’s post-test. **p < 0.01, ***p < 0.001 vs saline treatment; °°p < 0.01 vs 2 cm transplanted mice; ^{# # #}p < 0.001 vs 1 cm transplanted mice.