Figure 4. Reconstruction of the mitochondrial genome of P.saccamoebae with GC Content (represented by height of grey bars - black line is 50%) and gene annotations, which include the seven genes typically found in Fungi involved Complex I of oxidative phosphorylation.

Figure 4—figure supplement 1. Presence and absence of mitochondrial genes in P.saccamoebae and related species.

Presence of mitochondrial genes (x) are shown in the table with major loss of the seven NADH dehydrogenase genes highlighted in pink. Major losses of genes in Complex I of oxidative phosphorylation (large red X) and ribosomal proteins (with red strike through) are mapped onto the phylogenetic relationships (inferred from nuclear phylogeny, Figure 2). Hypothetical mitochondrial ribosomal protein content of the ancestor to Holomycota is inferred to have rps3, rps11, and rp12, which have been retained differentially across the three lineages shown.

Figure 4—figure supplement 2. Maximum likelihood phylogeny of Mitochondrial genes cox1, cox2, cox3, cob1, and atp6.

There were 1177 amino acid positions in the concatenated alignment, and the PROTGAMMAWAG model was used for phylogenetic reconstruction in RAxML.