Figure 6. P7C3-A20 stimulates NAD recovery in response to depletion with H₂O₂ treatment in vitro.

(A) Effects of vehicle, P7C3-A20 (3 μM), or NAM (1 mM) on intracellular NAD production under basal conditions. A1A1 cells were pre-treated with vehicle (30 min) followed by indicated treatment (60 min). (B and C) Effects of vehicle, P7C3-A20, or NAM (1 mM) on intracellular NAD production in response to pretreatment with H₂O₂ (200 μM, (B). FK866 (10 nM) was co-administered with P7C3-A20 (3 μM) or NAM (1 mM) after H₂O₂ pretreatment (C). Treatment conditions were performed in quadruplicate for each experiment. Bars represent mean NAD accumulation ±SEM expressed as a percentage of vehicle. Circles are mean NAD values from independent experiments. #p<0.05 vs. Veh/Veh or ***p<0.001, *p<0.05 vs. H₂O₂/Veh by one-way ANOVA with Dunnett’s post-hoc test, n = 4–5 independent experiments.